{"id":17992,"date":"2015-04-07T16:14:14","date_gmt":"2015-04-07T13:14:14","guid":{"rendered":"http:\/\/balarisi.org\/?p=17992"},"modified":"2015-04-07T23:20:17","modified_gmt":"2015-04-07T20:20:17","slug":"turk-propolis-ekstraktlarinin-iyonizan-y-gama-radyasyonla-uyarilmis-insan-lenfositleri-uzerine-olasi-radyoprotektif-etkisinin-degerlendirilmesi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17992","title":{"rendered":"T\u00fcrk propolis ekstraktlar\u0131n\u0131n iyonizan (y-Gama) radyasyonla uyar\u0131lm\u0131\u015f insan lenfositleri \u00fczerine olas\u0131 radyoprotektif etkisinin de\u011ferlendirilmesi"},"content":{"rendered":"

Can \u00d6zg\u00fcr Yal\u00e7\u0131n<\/strong>
\nY\u00fcksek Lisans Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ T\u0131bbi Biyokimya Anabilim Dal\u0131, 2012, 88 s.
\nTEZ NO: 341974<\/strong>
\nSorgu Penceresine TEZ NO\u2019yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n

\u00d6ZET
\n<\/strong> Propolis, polifenolik bile\u015fikler ve flavonoidler a\u00e7\u0131s\u0131ndan zengin, topland\u0131\u011f\u0131 b\u00f6lgenin co\u011frafyas\u0131na ve iklimine g\u00f6re kompozisyonu de\u011fi\u015febilen, antibakteriyel, antit\u00fcm\u00f6ral, antiinflamatuar, antioksidatif, antimutajenik ve di\u011fer faydal\u0131 aktiviteleri ile do\u011fal bir ila\u00e7 olarak kullan\u0131lan \u00f6nemli bir ar\u0131 \u00fcr\u00fcn\u00fcd\u00fcr. Propolisin biyolojik etkisi, \u00e7o\u011funlukla flavonoidlerin varl\u0131\u011f\u0131na atfedilmektedir. \u0130yonla\u015ft\u0131r\u0131c\u0131 radyasyona maruziyet canl\u0131larda do\u011frudan ve dolayl\u0131 genotoksik hasara yol a\u00e7ar. Do\u011frudan etki DNA \u00fczerinde tek ve \u00e7ift zincir k\u0131rklar\u0131 \u015feklinde olurken; dolayl\u0131 etki iyonla\u015ft\u0131r\u0131c\u0131 radyasyonun su ile etkile\u015fimi sonucu meydana gelen serbest radikaller \u00fczerinden ger\u00e7ekle\u015fir. Olu\u015fan bu serbest radikaller DNA, protein, lipid, karbonhidratlar gibi biyomolek\u00fcllerle etkile\u015fime girebilir ve oksidatif DNA hasar\u0131 sonucu mutajenik ve karsinojenik etki olu\u015fturabilirler. G\u00fcn\u00fcm\u00fczde genotoksisite \u00e7al\u0131\u015fmalar\u0131nda bir\u00e7ok y\u00f6ntem kullan\u0131lmakla birlikte; basit, ucuz ve tekrarlanabilir bir metot olan comet analizi di\u011fer y\u00f6ntemlerden bir ad\u0131m \u00f6ne \u00e7\u0131kmaktad\u0131r. Bu \u00e7al\u0131\u015fmada, T\u00fcrk propolisinin farkl\u0131 konsantrasyonlarda haz\u0131rlanm\u0131\u015f etanolik ekstraktlar\u0131n\u0131n 3 Gy gama radyasyon (30cGy\/dak.) ile uyar\u0131lm\u0131\u015f fibroblast h\u00fccre serileri \u00fczerindeki olas\u0131 radyoprotektif etkisi comet y\u00f6ntemi ile incelenmi\u015ftir. H\u00fccrelerin \u0131\u015f\u0131nlanmadan \u00f6nce 100, 200 ve 300 ?g\/mL\u2019lik konsantrasyonlardaki etanolik propolis ekstrakt\u0131 ile 15 ve 30 dakikal\u0131k \u00f6n muameleleri sonras\u0131nda elde comet skorlar\u0131 ile sadece 3 Gy hasarl\u0131 h\u00fccrelerden elde edilen de\u011ferler aras\u0131nda anlaml\u0131 fark g\u00f6r\u00fcld\u00fc (p<0.001). Pozitif kontrol grubu olu\u015fturmak i\u00e7in radyasyona kar\u015f\u0131 koruyucu etkinli\u011fi bilinen sentetik bir aminotiyol bile\u015fi\u011fi olan amifostin kullan\u0131ld\u0131. Sonu\u00e7 olarak T\u00fcrk propolisinin etanolik ekstraktlar\u0131n\u0131n; fibroblast h\u00fccre serilerinde, 3 Gy gama radyasyon kaynakl\u0131 DNA hasar\u0131n\u0131 engelledi\u011fi ve radyoprotektif etkinli\u011fe sahip olabilece\u011fi neticesine var\u0131ld\u0131.
\nAnahtar Kelimeler: <\/strong> Comet analizi; Flavonoidler; Propolis; Radyoprotektif ajanlar; \u0130yonizan radyasyon<\/p>\n","protected":false},"excerpt":{"rendered":"

Can \u00d6zg\u00fcr Yal\u00e7\u0131n Y\u00fcksek Lisans Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ T\u0131bbi Biyokimya Anabilim Dal\u0131, 2012, 88 s. TEZ NO: 341974<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17992","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-can-ozgur-yalcin","anahtarkelimeler-propolis","anahtarkelimeler-propolis-ve-dna-hasari","anahtarkelimeler-propolisin-faydalari"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17992"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17992\/revisions"}],"predecessor-version":[{"id":17993,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17992\/revisions\/17993"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17992"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}