{"id":17982,"date":"2015-04-07T16:11:32","date_gmt":"2015-04-07T13:11:32","guid":{"rendered":"http:\/\/balarisi.org\/?p=17982"},"modified":"2015-04-07T23:24:40","modified_gmt":"2015-04-07T20:24:40","slug":"turkiyedeki-bal-numunelerinde-bulunan-hidroksimetilfurfural-miktari-stabilitesi-ve-hidroksimetilfurfural-miktar-tayini-analitik-metod-validasyonu","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17982","title":{"rendered":"T\u00fcrkiye\u2019deki bal numunelerinde bulunan hidroksimetilfurfural miktar\u0131, stabilitesi ve hidroksimetilfurfural miktar tayini analitik metod validasyonu"},"content":{"rendered":"<p><strong>Celaleddin \u0130pek<\/strong><br \/>\nY\u00fcksek Lisans Tezi, Marmara \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Analitik Kimya Anabilim Dal\u0131, 2012, 87 s.<br \/>\nTEZ NO: <strong>310157<\/strong><br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO\u2019yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong> Bu \u00e7al\u0131\u015fmada, balda bulunan ve bala yap\u0131lan \u0131s\u0131l i\u015flemler, bal\u0131n uzun s\u00fcreli bekletilmesi ve saklama ko\u015fullar\u0131 sonucu ortaya \u00e7\u0131kan Hidroksimetilfurfural (HMF) i\u00e7eri\u011fi ve analiz s\u00fcresi boyunca stabilitesi incelenmi\u015ftir. Pazarda bulunan ve yerel olarak \u00fcretilen bal \u00f6rneklerinin HMF i\u00e7erikleri ters fazl\u0131 y\u00fcksek performansl\u0131 s\u0131v\u0131 kromatografisi ile tayin edilmi\u015ftir. \u00c7ok eski zamanlardan beri t\u00fcm k\u00fclt\u00fcrlerde kullan\u0131lan bal\u0131n bir\u00e7ok hastal\u0131\u011f\u0131 tedavi etmede alternatif ila\u00e7 olarak al\u0131nd\u0131\u011f\u0131 ve bir antioksidan kayna\u011f\u0131 oldu\u011fu, kalp krizi riskini azaltt\u0131\u011f\u0131, antikanserojen, ba\u011f\u0131\u015f\u0131kl\u0131k sistemini kuvvetlendirici, katarakt ve iltihap al\u0131c\u0131 \u00f6zellikleri gibi \u00e7ok geni\u015f farmakolojik etkisi oldu\u011fu bilinmektedir. HMF\u2019nin ise bal\u0131n kalitesini d\u00fc\u015f\u00fcrd\u00fc\u011f\u00fc ve insan sa\u011fl\u0131\u011f\u0131 \u00fczerine olumsuz etkisi oldu\u011fu d\u00fc\u015f\u00fcn\u00fclmektedir. \u00c7al\u0131\u015fmada ilk olarak se\u00e7ici, hassas ve tekrarlanabilirli\u011fi y\u00fcksek bir HPLC y\u00f6ntemi geli\u015ftirilmi\u015f ve analitik metot valide edilmi\u015ftir. Bir\u00e7ok dereceli ve izokratik el\u00fcsyon sistemi denenmi\u015f ve en uygun sistem bulunmu\u015ftur. Hareketli faz bile\u015fenleri olarak, A: Su (pH\u2019\u0131 H2SO4 ile 3,0\u2019a ayarlanm\u0131\u015f) ve B: Asetonitril (ACN) kullan\u0131lm\u0131\u015ft\u0131r. RP-HPLC sistemi: Phenomenex C18 4,6 mm x 250 mm, 5 \u00b5m kolon, ak\u0131\u015f h\u0131z\u0131 1mL \/ dakika, ?=285 nm dalga boyu ve diod dizi dedekt\u00f6r (DAD) kullan\u0131lm\u0131\u015ft\u0131r. HMF miktar\u0131na ve HMF analizine etki eden fakt\u00f6rler incelenmi\u015f, Carrez sol\u00fcsyonlar\u0131n\u0131n, \u0131s\u0131n\u0131n, \u0131\u015f\u0131\u011f\u0131n ve bekletilme s\u00fcrelerinin etkisi bulunmu\u015ftur. ICH\u2019e g\u00f6re Akdeniz ku\u015fa\u011f\u0131nda bulunan T\u00fcrkiye\u2019nin normal ko\u015fulu olan 25\u00b12 \u00baC s\u0131cakl\u0131k ve %60\u00b15 nem de\u011ferlerinde, ara ko\u015ful olan 30\u00b12 \u00baC s\u0131cakl\u0131k ve %65\u00b15 nem de\u011ferlerinde, h\u0131zland\u0131r\u0131lm\u0131\u015f ko\u015ful olan 40\u00b12 \u00baC s\u0131cakl\u0131k ve %75\u00b15 nem de\u011ferlerinde bekletilen numunelere 3 ayl\u0131k stabilite \u00e7al\u0131\u015fmas\u0131 yap\u0131lm\u0131\u015ft\u0131r. Pazarda bulunan 6 ve y\u00f6resel olarak \u00fcretilen 4 farkl\u0131 bal numunelerine, pazarda bulunan 1 re\u00e7el numunesine, y\u00f6resel olarak \u00fcretilen 1 re\u00e7el numunesine ve yine y\u00f6resel olarak \u00fcretilen 1 pekmez numunesine HMF analizi yap\u0131lm\u0131\u015ft\u0131r.<br \/>\n<strong>Anahtar Kelimeler: <\/strong> Bal; Hidroksimetilfurfural; Miktar tayini; Y\u00fcksek Performansl\u0131 S\u0131v\u0131 Kromatografisi (HPLC).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Celaleddin \u0130pek Y\u00fcksek Lisans Tezi, Marmara \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Analitik Kimya Anabilim Dal\u0131, 2012, 87 s. TEZ NO: 310157<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17982","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-celaleddin-ipek","anahtarkelimeler-bal","anahtarkelimeler-bal-analizi","anahtarkelimeler-bal-ozelliklerinin-karsilastirilmasi","anahtarkelimeler-bal-ve-hmf-sorunu","anahtarkelimeler-hmf"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17982","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17982"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17982\/revisions"}],"predecessor-version":[{"id":17983,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17982\/revisions\/17983"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17982"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17982"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17982"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}