{"id":17921,"date":"2015-04-06T23:18:13","date_gmt":"2015-04-06T20:18:13","guid":{"rendered":"http:\/\/balarisi.org\/?p=17921"},"modified":"2015-04-08T11:24:41","modified_gmt":"2015-04-08T08:24:41","slug":"propolis-ekstraktlarinin-fibroblast-hucre-serilerinde-h2o2-ile-uyarilmis-dna-hasari-genotoksisite-uzerine-etkisinin-comet-assay-yontemi-ile-arastirilmasi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17921","title":{"rendered":"Propolis ekstraktlar\u0131n\u0131n fibroblast h\u00fccre serilerinde H2O2 ile uyar\u0131lm\u0131\u015f DNA hasar\u0131 (Genotoksisite) \u00fczerine etkisinin comet assay y\u00f6ntemi ile ara\u015ft\u0131r\u0131lmas\u0131"},"content":{"rendered":"<p><strong>Selim Demir<\/strong><br \/>\nY\u00fcksek Lisans Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ T\u0131bbi Biyokimya Anabilim Dal\u0131, 2010, 83 s.<br \/>\nTEZ NO: <strong>266653<\/strong><br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO\u2019yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong> Propolis, polifenolik bile\u015fikler ve flavonoidler a\u00e7\u0131s\u0131ndan zengin, topland\u0131\u011f\u0131 b\u00f6lgeninco\u011frafyas\u0131na ve iklimine g\u00f6re kompozisyonu de\u011fi\u015febilen, antibakteriyel, antit\u00fcm\u00f6ral,antiinflamatuar, antioksidatif, antimutajenik ve di\u011fer faydal\u0131 aktiviteleri ile do\u011fal bir ila\u00e7olarak kullan\u0131lan \u00f6nemli bir ar\u0131 \u00fcr\u00fcn\u00fcd\u00fcr. Propolisin biyolojik etkisi, \u00e7o\u011funluklaflavonoidlerin varl\u0131\u011f\u0131na atfedilmektedir. Reaktif oksijen \u00fcr\u00fcnlerinin olu\u015fumundaki artma,antioksidan enzim d\u00fczeylerindeki azalma ve\/veya DNA onar\u0131m mekanizmalar\u0131nda defektolmas\u0131, oksidatif DNA hasar\u0131 olu\u015fumuna yol a\u00e7maktad\u0131r. Oksidatif hasara ba\u011fl\u0131 olarakDNA\u2019da; tek ve \u00e7ift zincir k\u0131r\u0131klar\u0131, alkali labil b\u00f6lgeler, baz modifikasyonlar\u0131 ve \u015feker-fosfatomurga hasar\u0131 meydana gelebilir veya DNA ile protein aras\u0131nda \u00e7apraz ba\u011flanma olabilir.G\u00fcn\u00fcm\u00fczde genotoksisite \u00e7al\u0131\u015fmalar\u0131nda bir\u00e7ok y\u00f6ntem kullan\u0131lmakla birlikte; basit, ucuz vetekrarlanabilir bir metot olan comet analizi di\u011fer y\u00f6ntemlerden bir ad\u0131m \u00f6ne \u00e7\u0131kmaktad\u0131r. Bu\u00e7al\u0131\u015fmada, fibroblast h\u00fccre serileri kullan\u0131larak Trabzon propolisinin etanolik ekstraktlar\u0131n\u0131n50 ?g\/mL\u2019 lik konsantrasyonda, H2O2 ile ind\u00fcklenmi\u015f DNA hasar\u0131 \u00fczerine etkisi cometanalizi ile incelendi. H\u00fccrelerin, 50 ?g\/mL etanolik propolis ekstrakt\u0131yla; genotoksik ajandan\u00f6nce, genotoksik ajanla ayn\u0131 anda ve genotoksik ajandan sonra muamelesi sonucu DNAhasar\u0131ndaki % azalma s\u0131ras\u0131yla; % 96, % 98.92 ve % 99.84 olarak saptand\u0131. Sonu\u00e7 olarakpropolisin etanolik ekstraktlar\u0131n\u0131n; fibroblast h\u00fccre serilerinde, H2O2 kaynakl\u0131 DNA hasar\u0131n\u0131engelledi\u011fi ve antigenotoksik aktiviteye sahip olabilece\u011fi sonucuna var\u0131ld\u0131.<br \/>\n<strong>Anahtar Kelimeler: <\/strong>Propolis, DNA comet analizi, Genotoksisite, Antigenotoksisite, Primerfibroblast h\u00fccre serileri.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Selim Demir Y\u00fcksek Lisans Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ T\u0131bbi Biyokimya Anabilim Dal\u0131, 2010, 83 s. TEZ NO: 266653<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17921","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-selim-demir","anahtarkelimeler-propolis","anahtarkelimeler-propolis-ve-dna-hasari","anahtarkelimeler-propolisin-faydalari"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17921"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17921\/revisions"}],"predecessor-version":[{"id":17922,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17921\/revisions\/17922"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17921"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17921"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}