{"id":17885,"date":"2015-04-06T23:06:42","date_gmt":"2015-04-06T20:06:42","guid":{"rendered":"http:\/\/balarisi.org\/?p=17885"},"modified":"2015-04-09T12:06:39","modified_gmt":"2015-04-09T09:06:39","slug":"ari-sutu-ve-buzagi-serumunun-sigir-oositlerinin-vitrifikasyonu-ve-olgunlasmasi-uzerine-etkilerinin-arastirilmasi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17885","title":{"rendered":"Ar\u0131 s\u00fct\u00fc ve buza\u011f\u0131 serumunun s\u0131\u011f\u0131r oositlerinin vitrifikasyonu ve olgunla\u015fmas\u0131 \u00fczerine etkilerinin ara\u015ft\u0131r\u0131lmas\u0131"},"content":{"rendered":"

Zeli\u0307ha Necla Aksu<\/strong>
\nY\u00fcksek Lisans Tezi, Mustafa Kemal \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2010, 65 s.
\nTEZ NO: 287572<\/strong>
\nSorgu Penceresine TEZ NO\u2019yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n

\u00d6ZET
\n<\/strong> Bu \u00e7al\u0131\u015fmayla ama\u00e7lanan; seruma alternatif olarak d\u00fc\u015f\u00fcn\u00fclen ar\u0131 s\u00fct\u00fcn\u00fcn oosit olgunla\u015ft\u0131rmada kullan\u0131larak, olgunla\u015ft\u0131r\u0131lan oositlerin OPS vitrifikasyon y\u00f6ntemiyle dondurulduktan sonra canl\u0131 kalma oranlar\u0131n\u0131 ve fertilizasyon yeteneklerinin ara\u015ft\u0131r\u0131lmas\u0131d\u0131r.\u00c7al\u0131\u015fman\u0131n materyalini Antakya Belediyesi\u2019ne ve \u00f6zel bir ki\u015fiye ait iki farkl\u0131 mezbahaneye kesim i\u00e7in getirilen Siyah-Beyaz Alaca s\u0131\u011f\u0131rlara ait ovaryumlardan elde edilen oositler olu\u015fturmaktad\u0131r. Oositler morfolojik olarak s\u0131n\u0131fland\u0131r\u0131lm\u0131\u015flar ve kaliteli olanlar in vitro olgunla\u015ft\u0131rma ortam\u0131na (TCM-199 + % 10 (v\/v) FBS + % 0,625 (w\/v) ar\u0131 s\u00fct\u00fc (AS5) + % 1,25 (w\/v) ar\u0131 s\u00fct\u00fc (AS4) + FSH (5 \u00b5g\/ml) + LH (5 \u00b5g\/ml) + 50 IU\/mL penisilin \/ 50 \u00b5g\/mL streptomisin) aktar\u0131larak, 38\u00b0C s\u0131cakl\u0131kta %5 CO2 li atmosferde 22-24 saat ink\u00fcbe edilmi\u015flerdir. Oositler ters mikroskop (Olympus, CKX41) alt\u0131nda X40 oran\u0131nda b\u00fcy\u00fct\u00fclerek n\u00fckleer olgunla\u015fmalar\u0131 incelenmi\u015f ve ters mikroskoba (Olympus, CKX41) ba\u011fl\u0131 floresan \u0131\u015f\u0131k kayna\u011f\u0131 ve UV filtre kullan\u0131larak olgunla\u015fmalar tespit edilmi\u015ftir. Olgunla\u015ft\u0131rma ortamlar\u0131na ilave edilen ar\u0131 s\u00fct\u00fcn\u00fcn kontrol grubunda kullan\u0131lan seruma alternatif olabilecek \u00f6l\u00e7\u00fcde oositlerin olgunla\u015fmas\u0131 (K: % 77,8, AS4: % 64,5 ve AS5: % 74,5) \u00fczerine etkili oldu\u011fu g\u00f6r\u00fclm\u00fc\u015ft\u00fcr (P>0,05). Ortama ilave edilen ar\u0131 s\u00fct\u00fc dondurulmu\u015f olgunla\u015fmayan oositlerin olgunla\u015fma oranlar\u0131n\u0131 olumlu y\u00f6nde etkilemi\u015f (HYC+K: % 71,4 HYC+V: % 54,3 AS5+K: % 61,2 AS5+V: % 60,8) fakat tespit edilen bu etki istatistiki olarak \u00f6nemli bulunmam\u0131\u015ft\u0131r. Ayr\u0131ca ar\u0131 s\u00fct\u00fc vitrifikasyon sonras\u0131 fertilizasyonu (AS5+K: % 54,7 ve AS5+V: % 62,5) olumlu \u015fekilde etkilemektedir.Sonu\u00e7 olarak, ar\u0131 s\u00fct\u00fc s\u0131\u011f\u0131r oositlerinin olgunla\u015ft\u0131r\u0131lmas\u0131 ve fertilizasyonunda seruma alternatif olma potansiyeline sahiptir. Ancak, ar\u0131 s\u00fct\u00fcn\u00fcn oosit vitrifikasyonu a\u00e7\u0131s\u0131ndan daha detayl\u0131 ara\u015ft\u0131r\u0131lmas\u0131na ihtiya\u00e7 vard\u0131r.
\nAnahtar Kelimeler: <\/strong> Ar\u0131 s\u00fct\u00fc; serum; s\u0131\u011f\u0131r oosit olgunla\u015fma; vitrifikasyon<\/p>\n","protected":false},"excerpt":{"rendered":"

Zeli\u0307ha Necla Aksu Y\u00fcksek Lisans Tezi, Mustafa Kemal \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2010, 65 s. TEZ NO: 287572<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17885","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-zeliha-necla-aksu","anahtarkelimeler-ari-sutu"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17885","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17885"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17885\/revisions"}],"predecessor-version":[{"id":17886,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17885\/revisions\/17886"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17885"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17885"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17885"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}