{"id":17823,"date":"2015-04-03T12:20:50","date_gmt":"2015-04-03T09:20:50","guid":{"rendered":"http:\/\/balarisi.org\/?p=17823"},"modified":"2015-04-09T12:20:52","modified_gmt":"2015-04-09T09:20:52","slug":"karabas-otu-lavandula-stoechas-l-bitkisinin-farkli-in-vitro-besin-ortamlarinda-kulture-alinmasi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17823","title":{"rendered":"Karaba\u015f otu (Lavandula stoechas L. ) bitkisinin farkl\u0131 in vitro besin ortamlar\u0131nda k\u00fclt\u00fcre al\u0131nmas\u0131"},"content":{"rendered":"

Serpil Orhan<\/strong>
\nY\u00fcksek Lisans Tezi, Ege \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Biyom\u00fchendislik Anabilim Dal\u0131, 2007, 63 s.
\nTEZ NO: 202043<\/strong>
\nSorgu Penceresine TEZ NO’yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n

\u00d6ZET
\n<\/strong> Bu tezde, t\u0131bbi ve aromatik \u00f6zelliklere sahip karaba\u015f otu bitkisine ait s\u00fcrg\u00fcn nodeksplantlar\u0131n\u0131n farkl\u0131 besin ortamlar\u0131ndaki b\u00fcy\u00fcme parametreleri incelenmi\u015ftir.\u00c7imlendirme, s\u00fcrg\u00fcn olu\u015fumu ve geli\u015fimi ile k\u00f6klendirme i\u00e7in uygun besinortamlar\u0131 belirlenmi\u015ftir. En iyi \u00e7imlendirmeler (%97), 1 mg\/L Benzil Amino P\u00fcrin (BAP)i\u00e7eren Murashige ve Skoog (1962) (MS) besin ortam\u0131nda ger\u00e7ekle\u015ftirilmi\u015ftir.\u00c7imlendirilen iki adet karaba\u015f otu tohumundan elde edilen s\u00fcrg\u00fcn nod eksplantlar\u0131ndan invitro bitki klonlar\u0131 (klon 1 ve klon 2) \u00e7o\u011falt\u0131lm\u0131\u015ft\u0131r. Klon 1 bitkiciklerinin s\u00fcrg\u00fcn nodeksplantlar\u0131 9 farkl\u0131 MS ortam\u0131na aktar\u0131lm\u0131\u015f ve b\u00fcy\u00fcme parametreleri; s\u00fcrg\u00fcn uzunlu\u011fu,s\u00fcrg\u00fcn say\u0131s\u0131, s\u00fcrg\u00fcndeki nod say\u0131s\u0131 ve k\u00f6k say\u0131s\u0131 ile kallus olu\u015fturan eksplant y\u00fczdesiincelenmi\u015ftir. Klon 1 bitkiciklerine ait s\u00fcrg\u00fcn nod eksplantlar\u0131nda, 0,1 mg\/L NaftalenAsetik Asit (NAA) (ortalama 3,6 s\u00fcrg\u00fcn\/eksplant, 8,44 nod\/eksplant) ve 0,5 mg\/L \u0130ndolB\u00fctirik asit (IBA) (ortalama 3,44 s\u00fcrg\u00fcn\/eksplant, 8,62 nod\/eksplant) i\u00e7eren MSortamlar\u0131nda en iyi s\u00fcrg\u00fcn geli\u015fimleri sa\u011flanm\u0131\u015ft\u0131r. En iyi k\u00f6klenmeler ise 1 mg\/L NAA(ortalama 3,44 k\u00f6k\/eksplant) ve 0,5 mg\/L NAA (ortalama 2,29 k\u00f6k\/eksplant) i\u00e7eren MSortamlar\u0131nda g\u00f6zlenmi\u015ftir. K\u00fclt\u00fcre al\u0131nan eksplantlarda en uzun boylu bitkicikler(ortalama 2,23 cm) 0,1 mg\/L IBA i\u00e7eren MS ortam\u0131nda elde edilmi\u015ftir. S\u00fcrg\u00fcn nodeksplantlar\u0131, 1 mg\/L BAP (%68,09) ve 0,5 mg\/L BAP (%37,77) i\u00e7eren MS ortam\u0131nday\u00fcksek oranda kallus olu\u015fturmu\u015flard\u0131r. Klon 2 bitkiciklerinden al\u0131nan s\u00fcrg\u00fcn nodeksplantlar\u0131 2 farkl\u0131 MS ortam\u0131na aktar\u0131lm\u0131\u015f ve elde edilen b\u00fcy\u00fcme parametreleri klon 1bitkilerinin b\u00fcy\u00fcme parametreleri ile kar\u015f\u0131la\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. B\u00fcy\u00fcme parametreleri i\u00e7inyap\u0131lan istatistiki de\u011ferlendirmede iki klon aras\u0131ndaki farkl\u0131l\u0131klar \u00f6nemsiz bulunmu\u015ftur.VIT\u00fcm k\u00fclt\u00fcrlerde, 4000 lux ayd\u0131nlatma, 27\u00b14\u00baC s\u0131cakl\u0131k ve 16 saat ayd\u0131nl\u0131k, 8 saatkaranl\u0131k fotoperiyot kullan\u0131lm\u0131\u015ft\u0131r.
\nAnahtar Kelimeler: <\/strong> Lavandula stoechas L., karaba\u015f otu, in vitro, klon, b\u00fcy\u00fcmeparametreleri.<\/p>\n","protected":false},"excerpt":{"rendered":"

Serpil Orhan Y\u00fcksek Lisans Tezi, Ege \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Biyom\u00fchendislik Anabilim Dal\u0131, 2007, 63 s. TEZ NO: 202043<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17823","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-serpil-orhan","anahtarkelimeler-ari-merasi","anahtarkelimeler-karabas-otu"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17823","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17823"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17823\/revisions"}],"predecessor-version":[{"id":17824,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17823\/revisions\/17824"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17823"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17823"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17823"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}