{"id":17753,"date":"2015-04-02T15:39:06","date_gmt":"2015-04-02T12:39:06","guid":{"rendered":"http:\/\/balarisi.org\/?p=17753"},"modified":"2015-04-09T15:01:37","modified_gmt":"2015-04-09T12:01:37","slug":"sigir-oositlerinin-in-vitro-nukleer-olgunlasmasi-uzerine-ari-sutunun-etkisi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17753","title":{"rendered":"S\u0131\u011f\u0131r Oositlerinin \u0130n vitro n\u00fckleer olgunla\u015fmas\u0131 \u00fczerine ar\u0131 s\u00fct\u00fcn\u00fcn etkisi"},"content":{"rendered":"<p><strong>Emre \u015eirin<\/strong><br \/>\nY\u00fcksek Lisans Tezi, Ondokuz May\u0131s \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2004, 76 s.<br \/>\nTEZ NO: <strong>155063<\/strong><br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO&#8217;yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong>Bu \u00e7al\u0131\u015fman\u0131n amac\u0131, s\u0131\u011f\u0131r oositlerinin in vitro olgunla\u015fmalar\u0131nda k\u00fclt\u00fcr ortam\u0131nda protein kayna\u011f\u0131 olarak serum yerine ar\u0131 s\u00fct\u00fcn\u00fcn kullan\u0131m olanaklar\u0131n\u0131n ara\u015ft\u0131r\u0131lmas\u0131d\u0131r. Ara\u015ft\u0131rman\u0131n materyalini, Samsun B\u00fcy\u00fck\u015fehir Belediyesi Mezbahas\u0131 (SAMES A.\u015e.)&#8217;\u0131nda kesilen s\u0131\u011f\u0131rlardan al\u0131nan ovaryumlardan elde edilen oositler olu\u015fturmu\u015ftur. Oositler, ovaryumlar \u00fczerinde bulunan 2-8 mm \u00e7ap\u0131ndaki follik\u00fcllerden aspire edilmi\u015ftir. Elde edilen oositler, protein kayna\u011f\u0131 olarak %10 ar\u0131 s\u00fct\u00fc veya %10 f\u00f6tal buza\u011f\u0131 serumu ilave edilen iki farkl\u0131 k\u00fclt\u00fcr ortam\u0131nda (bikarbonat tamponlu TCM-199) 39 \u00b0C&#8217;de, havada %5 C02 ve %95 nemli atmosferde 22 saat s\u00fcreyle olgunla\u015ft\u0131r\u0131lm\u0131\u015flard\u0131r. %10 ar\u0131 s\u00fct\u00fc ilave edilen k\u00fclt\u00fcr ortam\u0131ndaki in vitro olgunla\u015fma oran\u0131 %78 (Metefaz-ll&#8217;ye ula\u015fanlar\u0131n oran\u0131) iken, f\u00f6tal buza\u011f\u0131 serumu ilave edilen k\u00fclt\u00fcr ortam\u0131nda bu oran %77 olarak tespit edilmi\u015ftir (P&gt;0.05). \u0130n vitro olgunla\u015fma sonras\u0131 parthenogenetik aktivasyonu takiben 48. saate kadar, protein kayna\u011f\u0131 olarak %10 ar\u0131 s\u00fct\u00fc ilave edilen k\u00fclt\u00fcr ortam\u0131nda olgunla\u015ft\u0131rman oositlerin %36&#8217;s\u0131, f\u00f6tal buza\u011f\u0131 serumu ilave edilen k\u00fclt\u00fcr ortam\u0131nda olgunla\u015ft\u0131rman oositlerin ise %42&#8217;si granulosa monolayer h\u00fccre k\u00fclt\u00fcr\u00fc ile ko-k\u00fclt\u00fcrde 2 h\u00fccreli ve daha ileriki a\u015famalara kadar b\u00f6l\u00fcnm\u00fc\u015ft\u00fcr (P&gt;0.05). Sonu\u00e7 olarak, protein kayna\u011f\u0131 olarak k\u00fclt\u00fcr ortam\u0131na %10 ar\u0131 s\u00fct\u00fc ilavesinin s\u0131\u011f\u0131r oositlerinin in vitro olgunla\u015fmalar\u0131nda ba\u015far\u0131l\u0131 bir \u015fekilde kullan\u0131labilece\u011fi ortaya konulmu\u015ftur.<br \/>\n<strong>Anahtar Kelimeler: <\/strong>S\u0131\u011f\u0131r, oosit, in vitro, n\u00fckleer olgunla\u015fma, ar\u0131 s\u00fct\u00fc, parthenogenetik aktivasyon<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Emre \u015eirin Y\u00fcksek Lisans Tezi, Ondokuz May\u0131s \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2004, 76 s. TEZ NO: 155063<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17753","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-emre-sirin","anahtarkelimeler-ari-sutu"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17753","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17753"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17753\/revisions"}],"predecessor-version":[{"id":17754,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17753\/revisions\/17754"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17753"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17753"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17753"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}