{"id":17714,"date":"2015-04-01T11:21:56","date_gmt":"2015-04-01T08:21:56","guid":{"rendered":"http:\/\/balarisi.org\/?p=17714"},"modified":"2015-04-09T15:07:12","modified_gmt":"2015-04-09T12:07:12","slug":"buyuk-mum-guvesi-galleria-mellonella-l-ya-etkili-bacillus-thuringiensis-suslari-ve-bunlarin-plazmid-icerikleri-uzerinde-arastirmalar","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17714","title":{"rendered":"B\u00fcy\u00fck mum g\u00fcvesi (Galleria mellonella L.) ya etkili Bacillus thuringiensis su\u015flar\u0131 ve bunlar\u0131n plazmid i\u00e7erikleri \u00fczerinde ara\u015ft\u0131rmalar"},"content":{"rendered":"

Mustafa Ak\u00e7elik<\/strong>
\nY\u00fcksek Lisans Tezi, Ankara \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Tar\u0131m \u00dcr\u00fcnleri Teknolojisi Anabilim Dal\u0131, 1987, 63 s.
\nTEZ NO:\u00a01149<\/strong>
\nSorgu Penceresine TEZ NO’yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n

\u00d6ZET
\n<\/strong>Bu \u00e7al\u0131\u015fmada bal ar\u0131s\u0131 kovanlar\u0131ndan alman \u00f6l\u00fc b\u00fcy\u00fck mum g\u00fcvesi larvalar\u0131ndan izole edilen El izolat\u0131 ile Sandoz (\u0130svi\u00e7re) taraf\u0131ndan \u00fcretilen Thuriap adl\u0131 pre- parattan izole edilen Bacillus thurinpiensis var, aizavvai susu kullan\u0131lm\u0131\u015ft\u0131r. Bl izolat\u0131n\u0131n te\u015fhisi sonucu Bacillus thuringiensis var. thuringiensis oldu\u011funa karar verilmi\u015ftir. Eu iki Bacillus thuringiensis t\u00fcr\u00fcn\u00fcn un r\u00fcvesi (Eohe stie kuehr.iella Zell) ve b\u00fcy\u00fck mum g\u00fcvesi (Galleria mellonella L. ) larva lar\u0131na kar\u015f\u0131 etkinlikleri incelenmi\u015ftir.\u000bIX Paha sonra kimyasal mutagenler olan Ethidium bromide ye Acridine orange ile mutasyona u\u011frat\u0131lan bakteriler Minimal agar \u00fczerinde koloni morfolojisi, amino asit ve karbon kay na\u011f\u0131 kullan\u0131m \u00f6zelliklerine g\u00f6re se\u00e7ilmi\u015ftir. Bunlara ila veten ana su\u015flar ile mutant su \u015fiardan baz\u0131lar\u0131n\u0131n antibiyo tik diren\u00e7lilik \u00f6zellikleri belirlenmi\u015ftir. Elde edilen 11 mutant su\u015f faz kontrast mikroskopta kristal protein ve spor olu\u015fturma yetenekleri y\u00f6n\u00fcnde ince lenmi\u015ftir. 11 lHutant susun \u00fc\u00e7\u00fcn\u00fcn spor olu\u015fturan\u00bb kristal olu\u015fturamayan di\u011ferlerinin ise kristal ve spor negatif olduklar\u0131 saptanm\u0131\u015ft\u0131r. Bunu takiben 11 mutant susun Ephestia kuehniella Zell ve galleria mellonella (L. ) larvalar\u0131na kar\u015f\u0131 etki leri belirlenmi\u015ftir. Kristal sentezinin plazmidde bulunan genler tara f\u0131ndan kontrol edildi\u011fi bilinmektedir. Bu nedenle bakterilerin plazmid profilleri incelenmi\u015ftir. Sonu\u00e7ta mutagen uygulamas\u0131 ile, plazmid bantlar\u0131nda de\u011fi\u015fiklik meydana gelmemi\u015f olma s\u0131na ra\u011fmen etkinin $ 10-30 a d\u00fc\u015ft\u00fc\u011f\u00fc saptanm\u0131\u015ft\u0131r. Elde edilen veriler Bacillus thuringiensis \u00fcretimi s\u0131ras\u0131nda kalite kontrol\u00fcnde kullan\u0131labilecek, plazmid profili ve faz kontrast mikroskopta kristal tespitini i\u00e7e ren bir y\u00f6ntem ortaya konmas\u0131na olanak sa\u011flam\u0131\u015ft\u0131r.
\nAnahtar Kelimeler: <\/strong> Bacillus thuringiensis, Plazmid, B\u00fcy\u00fck mum g\u00fcvesi, Kristal protein.<\/p>\n","protected":false},"excerpt":{"rendered":"

Mustafa Ak\u00e7elik Y\u00fcksek Lisans Tezi, Ankara \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Tar\u0131m \u00dcr\u00fcnleri Teknolojisi Anabilim Dal\u0131, 1987, 63 s. TEZ NO:\u00a01149<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[37],"tags":[],"class_list":["post-17714","post","type-post","status-publish","format-standard","","category-yuksek-lisans-tezleri","yazarlar-mustafa-akcelik","anahtarkelimeler-ari-zararlilari","anahtarkelimeler-mum-guvesi"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17714","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17714"}],"version-history":[{"count":3,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17714\/revisions"}],"predecessor-version":[{"id":17717,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17714\/revisions\/17717"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17714"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17714"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17714"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}