{"id":17602,"date":"2014-10-22T23:36:51","date_gmt":"2014-10-22T20:36:51","guid":{"rendered":"http:\/\/balarisi.org\/?p=17602"},"modified":"2015-03-30T21:54:32","modified_gmt":"2015-03-30T18:54:32","slug":"nitrik-oksit-sentaz-blokaji-ile-hipertansiyon-olusturulan-sicanlarda-propolis-cape-ve-polenin-kan-basinci-adma-nf-%ce%bab-ve-paraoksanaz-duzeylerine-etkileri","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17602","title":{"rendered":"Nitrik oksit sentaz blokaj\u0131 ile hipertansiyon olu\u015fturulan s\u0131\u00e7anlarda propolis, CAPE ve polen&#8217;in kan bas\u0131nc\u0131, ADMA, NF-\u03baB ve paraoksanaz d\u00fczeylerine etkileri"},"content":{"rendered":"<p><strong>Mehmet Fuat G\u00fclhan<\/strong><br \/>\nDoktora Tezi, Ni\u011fde \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Biyoloji Anabilim Dal\u0131, 2014, 129 s.<br \/>\nTEZ NO: 351991<br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO&#8217;yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong>N\u03c9-nitro-L-arjinin-metil ester (L-NAME) ile hipertansif yap\u0131lan s\u0131\u00e7anlarda propolis, kafeik asit fenil ester (CAPE) ve polen uygulamas\u0131 sonucu paraoksanaz (PON1) aktivitesi, toplam antioksidan seviye (TAS), toplam oksidan seviye (TOS), oksidatif stres indeksi (OS\u0130), asimetrik dimetilarjinin (ADMA) ve n\u00fckleer fakt\u00f6r kappa B (NF-\u03baB) seviyeleri ve kan bas\u0131nc\u0131nda meydana gelen de\u011fi\u015fimler ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Deneysel ara\u015ft\u0131rmalar i\u00e7in 35 adet Spraque dawley \u0131rk\u0131 s\u0131\u00e7anlar kullan\u0131lm\u0131\u015ft\u0131r. \u00c7al\u0131\u015fmada; kontrol (0.5 cc serum fizyolojik), hipertansiyon (L-NAME=HT) (40 mg\/kg\/g\u00fcn), HT+propolis (200 mg\/kg\/g\u00fcn), HT+polen (100 mg\/kg\/g\u00fcn) ve HT+CAPE (50 \u00b5M\/kg\/g\u00fcn) olmak \u00fczere 5 deneysel grup belirlenmi\u015f olup 28 g\u00fcn boyunca kontrol grubu d\u0131\u015f\u0131nda b\u00fct\u00fcn deneysel gruplara L-NAME uygulamas\u0131 devam etmi\u015ftir. 28 g\u00fcnl\u00fck uygulaman\u0131n ilk 14 g\u00fcn\u00fcnde kontrol d\u0131\u015f\u0131ndaki gruplara sadece L-NAME, 14. g\u00fcnden sonra L-NAME grubu hari\u00e7 di\u011fer gruplara antioksidan maddeler belirlenen dozlarla uygulanm\u0131\u015ft\u0131r. \u00c7al\u0131\u015fman\u0131n 0, 14 ve 28. g\u00fcnlerinde deney hayvanlar\u0131n\u0131n kan bas\u0131n\u00e7lar\u0131 tail cuff y\u00f6ntemiyle \u00f6l\u00e7\u00fclm\u00fc\u015ft\u00fcr. 28. g\u00fcn sonunda dekapite edilen s\u0131\u00e7anlar\u0131n kan, kalp ve b\u00f6brek doku numuneleri al\u0131n\u0131p bu dokularda PON1 aktivitesi, TAS, TOS, OS\u0130, ADMA ve NF-\u03baB seviyeleri \u00f6l\u00e7\u00fcld\u00fc ve veriler istatistiksel (P&lt;0.05) olarak de\u011ferlendirildi. Uygulaman\u0131n 14. g\u00fcn\u00fcnde L-NAME uygulama gruplar\u0131n\u0131n (HT) kan bas\u0131nc\u0131 de\u011ferleri kontrol grubu verilerine g\u00f6re anlaml\u0131 olarak (P&lt;0.05) y\u00fckselirken, 14. g\u00fcnden sonra propolis, CAPE ve polen uygulanan grupta kan bas\u0131nc\u0131 sonu\u00e7lar\u0131 HT grubuna g\u00f6re d\u00fc\u015f\u00fc\u015f g\u00f6sterdi (P&lt;0.05). S\u0131\u00e7an serumlar\u0131n\u0131n PON1 aktiviteleri HT grubunda kontrole g\u00f6re azal\u0131rken (P&lt;0.05), L-NAME ile birlikte uygulanan propolis, CAPE ve polen gruplar\u0131 HT grubu ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda anlaml\u0131 olarak artt\u0131 (P&lt;0.05). Kalp dokusu PON1 seviyeleri L-NAME uygulanan grupta kontrol grubuna g\u00f6re anlaml\u0131 olarak azal\u0131rken (P&lt;0.05), antioksidan takviyesi yap\u0131lan gruplar HT grubu ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda; CAPE ilavesi yap\u0131lan grubun PON1 aktivitesinde anlaml\u0131 bir de\u011fi\u015fimin olmad\u0131\u011f\u0131 (P&gt;0.05), propolis ve polen verilen gruplar\u0131n PON1 seviyelerinin istatistiksel olarak art\u0131\u015f (P&lt;0.05) g\u00f6sterdi\u011fi belirlenmi\u015ftir. B\u00f6brek dokusunda HT grubu PON1 seviyeleri kontrol grubuna g\u00f6re anlaml\u0131 olarak azalm\u0131\u015ft\u0131r (P&lt;0.05). Propolis, CAPE ve polen ilavesi yap\u0131lan gruplar\u0131n PON1 aktiviteleri, HT grubuna g\u00f6re anlaml\u0131 art\u0131\u015flar g\u00f6stermi\u015ftir (P&lt;0.05). S\u0131\u00e7an dokular\u0131nda; TAS de\u011ferleri HT grubunda kontrole g\u00f6re istatistiksel olarak azal\u0131rken (P&lt;0.05), antioksidan maddelerin eklendi\u011fi gruplarda ise HT grubuna g\u00f6re anlaml\u0131 art\u0131\u015flar (P&lt;0.05) saptand\u0131. TOS verileri ise HT grubunda kontrole g\u00f6re en y\u00fcksek seviyelere (P&lt;0.05) ula\u015f\u0131rken, antioksidan takviyesi yap\u0131lan gruplarda bu seviyelerin d\u00fc\u015f\u00fc\u015f (P&lt;0.05) g\u00f6sterdi\u011fi g\u00f6zlendi. Kan, kalp ve b\u00f6brek dokular\u0131ndaki ADMA ve NF-\u03baB seviyeleri; HT grubunda kontrol grubu verilerine g\u00f6re anlaml\u0131 art\u0131\u015flar (P&lt;0.05) g\u00f6sterdi. HT ile birlikte antioksidan ajanlar\u0131n uyguland\u0131\u011f\u0131 gruplarda ADMA ve NF-\u03baB de\u011ferleri, sadece L-NAME uygulanan grup ile kar\u015f\u0131la\u015ft\u0131r\u0131ld\u0131\u011f\u0131nda anlaml\u0131 olarak azald\u0131 (P&lt;0.05). Literat\u00fcr ara\u015ft\u0131rmalar\u0131na g\u00f6re, L-NAME ile NOS inhibisyonu olu\u015fturulan s\u0131\u00e7anlarda propolis ve CAPE&#8217;nin kan bas\u0131nc\u0131 \u00fczerine etkilerini belirlemek amac\u0131yla yap\u0131lm\u0131\u015f \u00e7al\u0131\u015fmalara rastlan\u0131rken polen ekstrakt\u0131 uygulamas\u0131 ile ilgili bir \u00e7al\u0131\u015fmaya rastlanmad\u0131. Y\u00fckselen kan bas\u0131nc\u0131n\u0131n d\u00fczenlenmesinde polen ve propolis ekstrakt\u0131n\u0131n daha etkili terap\u00f6tik maddeler olmas\u0131 sebebiyle, kimyasal olarak elde edilen CAPE yerine do\u011fal ar\u0131 \u00fcr\u00fcnlerinin kullan\u0131m\u0131 \u00f6nerilebilir. Uygulanan antioksidan etkili ar\u0131 \u00fcr\u00fcnlerinin hipertansiyon sonucu meydana gelen inflamasyon yola\u011f\u0131n\u0131n i\u015fleyi\u015fini engelledi\u011fi yap\u0131lan \u00e7al\u0131\u015fma ile ortaya konulmu\u015ftur. L-NAME ile hipertansif yap\u0131lan deney hayvanlar\u0131na uygulanan do\u011fal ar\u0131 \u00fcr\u00fcnleri olan propolis ve polenin kan, kalp ve b\u00f6brek dokular\u0131ndaki PON1 aktivitesini artt\u0131r\u0131c\u0131 etkiler g\u00f6sterdi\u011fi ilk kez bu \u00e7al\u0131\u015fma ile ortaya konmu\u015ftur.<\/p>\n<p><strong>Anahtar Kelimeler: <\/strong>Hipertansiyon, L-NAME, Paraoksanaz, Propolis, Kafeik asit fenil ester, Polen, Kan bas\u0131nc\u0131, Total antioksidan seviye, Total oksidan seviye, Oksidatif stres indeksi, Asimetrik dimetilarjinin, N\u00fckleer fakt\u00f6r kappa B<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Mehmet Fuat G\u00fclhan Doktora Tezi, Ni\u011fde \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Biyoloji Anabilim Dal\u0131, 2014, 129 s. TEZ NO: 351991<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[36],"tags":[163,358,115],"class_list":["post-17602","post","type-post","status-publish","format-standard","","category-doktora-tezleri","tag-apiterapi-2","tag-mehmet-fuat-gulhan","tag-propolis","yazarlar-mehmet-fuat-gulhan","anahtarkelimeler-apiterapi","anahtarkelimeler-propolis","anahtarkelimeler-propolisin-faydalari"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17602","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17602"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17602\/revisions"}],"predecessor-version":[{"id":17603,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17602\/revisions\/17603"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17602"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17602"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17602"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}