{"id":17565,"date":"2014-10-21T23:25:57","date_gmt":"2014-10-21T20:25:57","guid":{"rendered":"http:\/\/balarisi.org\/?p=17565"},"modified":"2015-03-30T22:10:35","modified_gmt":"2015-03-30T19:10:35","slug":"ari-poleni-ve-propolisinin-metastatik-insan-prostat-kanseri-hucre-serilerinde-voltaj-kapili-sodyum-kanallari-ekspresyonu-uzerine-etkisi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17565","title":{"rendered":"Ar\u0131 poleni ve propolisinin metastatik insan prostat kanseri h\u00fccre serilerinde voltaj kap\u0131l\u0131 sodyum kanallar\u0131 ekspresyonu \u00fczerine etkisi"},"content":{"rendered":"<p><strong>Meltem \u00c7olak<\/strong><br \/>\nDoktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2009, 111 s.<br \/>\nTEZ NO: 266646<br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO&#8217;yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong>Polen ve propolis anti-inflamatuar, antioksidan, antit\u00fcm\u00f6ral gibi \u00f6zelliklere sahip\u000bolan, topland\u0131\u011f\u0131b\u00f6lgenin co\u011frafyas\u0131na ve iklimine ba\u011fl\u0131olarak kompozisyonu de\u011fi\u015fen,\u000bflavonoidler ve polifenolik bile\u015fiklerce zengin ar\u0131\u00fcr\u00fcnleridir. Voltaj kap\u0131l\u0131sodyum kanal\u000bproteini(VGSC) h\u00fccrelere Na+ ak\u0131\u015f\u0131n\u0131 sa\u011flayan, metastatik kanser h\u00fccrelerinde\u000bekspresyonu ve aktivitesi olduk\u00e7a artan, ?ve \u00df izoformlar\u0131ndan olu\u015fan bir membran\u000bproteinidir. Bu \u00e7al\u0131\u015fmada, canl\u0131l\u0131k testi sonucu belirlenen T\u00fcrkiye poleni ve Trabzon\u000bpropolisinin DMSO ve sulu ekstraktlar\u0131n\u0131n s\u0131ras\u0131yla 150?g\/ml&#8217;lik ve 20?g\/ml&#8217;lik\u000bkonsantrasyonlar\u0131n\u0131n metastatik PC-3 prostat kanser h\u00fccrelerinde VGSC&#8217;nin Nav 1.5 ve\u000bNav 1.7 ?izoformlar\u0131ekspresyonu \u00fczerine etkisi RTPCR(Real Time PCR) \u00e7al\u0131\u015fmalar\u0131yla\u000bincelendi. PC-3 kanser h\u00fccreleriyle polen ve propolis ekstraktlar\u013124 saat ink\u00fcbe edildi.\u000b0mg\/ml ekstraktl\u0131PC-3 h\u00fccrelerinde VGSC ekspresyonu %100 kabul edildi. RTPCR\u000b\u00e7al\u0131\u015fmalar\u0131sonucunda kontrol h\u00fccrelerine g\u00f6re Nav 1.5 ve Nav 1.7 izoformlar\u0131n\u0131n gen\u000bekspresyonundaki en y\u00fcksek bask\u0131lanma 150?g\/ml&#8217;lik polen DMSO ektraktlar\u0131nca\u000bs\u0131ras\u0131yla %55.98 ve %53.31 oran\u0131nda, en d\u00fc\u015f\u00fck bask\u0131lanma ise 20?g\/ml&#8217;lik propolisin sulu\u000bekstraktlar\u0131nca s\u0131ras\u0131yla %18.01 ve %22.58 oran\u0131nda oldu\u011fu saptand\u0131. Sonu\u00e7 olarak polen\u000bve propolisin DMSO ve sulu ektraklar\u0131n\u0131n PC-3 prostat kanseri h\u00fccre serilerinde VGSC\u000bNav 1.5 ve Nav 1.7 ?izoformlar\u0131n\u0131n mRNA ekspresyonlar\u0131n\u0131de\u011fi\u015fik oranlarda bask\u0131lad\u0131\u011f\u0131\u000bve anti-metastatik aktiviteye sahip oldu\u011fu tespit edildi.<\/p>\n<p><strong>Anahtar Kelimeler: <\/strong>Polen, Propolis, Voltaj Kap\u0131l\u0131Sodyum Kanal Proteini, PC-3 Prostat\u000bKanser H\u00fccre Serisi<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Meltem \u00c7olak Doktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2009, 111 s. TEZ NO: 266646<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[36],"tags":[163,331,44,361,115,360,330],"class_list":["post-17565","post","type-post","status-publish","format-standard","","category-doktora-tezleri","tag-apiterapi-2","tag-meltem-colak","tag-polen","tag-polenin-faydalari","tag-propolis","tag-propolis-faydalari","tag-prostat-kanseri","yazarlar-meltem-colak","anahtarkelimeler-apiterapi","anahtarkelimeler-polen","anahtarkelimeler-polenin-faydalari","anahtarkelimeler-propolis","anahtarkelimeler-propolisin-faydalari","anahtarkelimeler-prostat-kanseri"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17565","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17565"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17565\/revisions"}],"predecessor-version":[{"id":17566,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17565\/revisions\/17566"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17565"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17565"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17565"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}