{"id":17563,"date":"2014-10-21T23:23:30","date_gmt":"2014-10-21T20:23:30","guid":{"rendered":"http:\/\/balarisi.org\/?p=17563"},"modified":"2015-03-30T22:11:38","modified_gmt":"2015-03-30T19:11:38","slug":"turk-propolisi-ekstraktlarinin-prostat-kanser-hucre-serilerinin-proteomigine-etkisi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17563","title":{"rendered":"T\u00fcrk propolisi ekstraktlar\u0131n\u0131n prostat kanser h\u00fccre serilerinin proteomi\u011fine etkisi"},"content":{"rendered":"<p><strong>Ya\u015fam Barlak<\/strong><br \/>\nDoktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2009, 108 s.<br \/>\nTEZ NO: 266648<br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO&#8217;yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong>Propolis, flavonoid ve fenolik bile\u015fikler a\u00e7\u0131s\u0131ndan zengin olan bir ar\u0131 \u00fcr\u00fcn\u00fcd\u00fcr. \u00c7e\u015fitli farmakolojik aktivitelere sahip olan propolisin kompozisyonu, topland\u0131\u011f\u0131 b\u00f6lgedeki yerel bitki \u00f6rt\u00fcs\u00fcne, mevsim ve iklime ba\u011fl\u0131 olarak \u00e7e\u015fitlilik g\u00f6sterir. Propolisin farmakolojik aktivitesi, \u00e7o\u011funlukla flavonoidlerin varl\u0131\u011f\u0131na atfedilmektedir. Proteom, h\u00fccrenin intrinsik genetik program\u0131 ve kendi yak\u0131n \u00e7evrelerindeki etkilerin her ikisini de yans\u0131tan proteinlerin dinamik bir kolleksiyonudur. Y\u00fczey ile geli\u015ftirilmi\u015f lazer desopsiyon iyonizasyon u\u00e7u\u015f zaman\u0131 k\u00fctle spektrometrisi (SELDI-TOF-MS), kanser proteomiks \u00e7al\u0131\u015fmalar\u0131nda yayg\u0131n olarak kullan\u0131lan bir proteomik yakla\u015f\u0131md\u0131r. Bu \u00e7al\u0131\u015fmada, T\u00fcrk propolisinin DMSO ve sulu ekstraktlar\u0131n\u0131n canl\u0131l\u0131k testi sonucu belirlenen 20\u00b5g\/ml&#8217;lik konsantrasyonlar\u0131n\u0131n PC-3 prostat kanser h\u00fccre serilerinin proteomi\u011fi \u00fczerine etkisi SELDI-TOF-MS ile incelendi. PC-3 kanser h\u00fccre serileri, propolis ekstraktlar\u0131 ile 24 saat ink\u00fcbe edildi. Elde edilen h\u00fccre lizatlar\u0131 CM10 ve Q10 Protein \u00e7ip dizinlerine y\u00fcklenerek, SELDI-TOF-MS&#8217;de analiz edildi. Kontrol h\u00fccreleri ile % 0,008 DMSO \u00e7\u00f6zeltisi ve propolisin DMSO ekstrakt\u0131 ile ink\u00fcbe edilen h\u00fccreler aras\u0131nda CM10 protein \u00e7ipi ile 14.424, 33.303, 32.800, 20.709, 27.294, 21.139, 5.141 ve 12.663 Da piklerinde; Q10 protein \u00e7ipi ile 61.257, 108.529, 144.776, 84.341 ve 85.994 Da piklerinde farkl\u0131 ekspresyon g\u00f6zlendi. Kontrol h\u00fccreleri ve sulu ekstrakt ile ink\u00fcbe edilen h\u00fccreler aras\u0131nda CM10 protein \u00e7ipi ile 6.268, 16.042, 24.664, 24.810 ve 32.043 Da piklerinde; Q10 protein \u00e7ipi ile 10.350, 11.603, 84. 348, 87.529, 89.004, 102.007, 144.762, 13.575, 85.989, 94.605, 104.407 ve 10.891 Da piklerinde farkl\u0131 ekspresyon g\u00f6zlendi. Propolisin DMSO&#8217;lu ve sulu ekstraktlar\u0131n\u0131n PC-3 kanser h\u00fccre serilerinde protein ekspresyon profilini de\u011fi\u015ftirerek anti-proliferatif aktiviteye sahip olabilece\u011fi sonucuna var\u0131ld\u0131.<\/p>\n<p><strong>Anahtar Kelimeler: <\/strong>Propolis, PC-3 prostat kanser h\u00fccre serileri, proteom, SELDI-TOF-MS<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ya\u015fam Barlak Doktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2009, 108 s. TEZ NO: 266648<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[36],"tags":[163,115,362,330,329],"class_list":["post-17563","post","type-post","status-publish","format-standard","","category-doktora-tezleri","tag-apiterapi-2","tag-propolis","tag-propolisin-faydalari","tag-prostat-kanseri","tag-yasam-barlak","yazarlar-yasam-barlak","anahtarkelimeler-apiterapi","anahtarkelimeler-propolis","anahtarkelimeler-propolisin-faydalari","anahtarkelimeler-prostat-kanseri"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17563","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17563"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17563\/revisions"}],"predecessor-version":[{"id":17564,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17563\/revisions\/17564"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17563"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17563"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17563"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}