{"id":17540,"date":"2014-09-28T23:09:30","date_gmt":"2014-09-28T20:09:30","guid":{"rendered":"http:\/\/balarisi.org\/?p=17540"},"modified":"2015-03-30T22:18:16","modified_gmt":"2015-03-30T19:18:16","slug":"mitokondriyel-dna-pcr-rflp-restriksiyon-parca-uzunluk-polimorfizmi-markerleri-kullanilarak-turkiyenin-farkli-yorelerine-ait-bal-arilarinin-tanimlanmasi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17540","title":{"rendered":"Mitokondriyel DNA PCR-RFLP (restriksiyon par\u00e7a uzunluk polimorfizmi) markerleri kullan\u0131larak T\u00fcrkiye’nin farkl\u0131 y\u00f6relerine ait bal ar\u0131lar\u0131n\u0131n tan\u0131mlanmas\u0131"},"content":{"rendered":"

Fulya \u00d6zdil<\/strong>
\nDoktora Tezi, Ankara \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2007, 141 s.
\nTEZ NO: 213800
\nSorgu Penceresine TEZ NO’yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n

\u00d6ZET
\n<\/strong>Bu arast\u0131rmada, T\u00fcrkiye bal ar\u0131s\u0131 populasyonlar\u0131 18 farkl\u0131 mitokondriyel DNA (mtDNA)\u000bmarkeri bak\u0131m\u0131ndan tan\u0131mlanm\u0131st\u0131r. T\u00fcrkiye’nin 20 farkl\u0131 y\u00f6resinden toplam 244 is\u00e7i ar\u0131\u000b\u00f6rnegi materyal olarak kullan\u0131lm\u0131st\u0131r. Bal ar\u0131s\u0131 populasyonlar\u0131n\u0131n tan\u0131mlanmas\u0131nda\u000brestriksiyon par\u00e7a uzunluk polimorfizmi (RFLP) ve DNA dizi analizi y\u00f6ntemlerinden\u000byararlan\u0131lm\u0131st\u0131r.\u000bMitokondriyel genomda anonim, sitokrom b (cyt b), sitokrom C oksidaz I (COI), ribozomun\u000bb\u00fcy\u00fck alt birimi (lrRNA), sitokrom C oksidaz I ile sitokrom C oksidaz II aras\u0131ndaki b\u00f6lge\u000b(COI-COII aras\u0131) ve sitokrom C oksidaz I ile sitokrom C oksidaz II intergenik b\u00f6lge (COICOII\u000bintergenik b\u00f6lge) dikkate al\u0131nm\u0131st\u0131r. \u00c7al\u0131smada anonim lokus PCR ile \u00e7ogalt\u0131lm\u0131st\u0131r. Cyt\u000bb lokusu BglII enzimi, COI lokusu HincII ve HinfI enzimleri, lrRNA lokusu EcoRI enzimi,\u000bCOI-COII aras\u0131ndaki b\u00f6lge XbaI enzimi ve COI-COII intergenik b\u00f6lge DraI enzimleriyle\u000bmuamele sonucu olusan 6 adet RFLP markeri ile anonim lokus ve COI-COII intergenik\u000bb\u00f6lgenin dizi analizi sonucunda tespit edilen 11 adet n\u00fckleotid farkl\u0131l\u0131g\u0131 olmak \u00fczere toplam\u000b18 farkl\u0131 mtDNA markeri \u00fczerinde durulmustur.\u000bT\u00fcrkiye bal ar\u0131s\u0131 populasyonlar\u0131 6 farkl\u0131 RFLP markeri ve anonim lokus bak\u0131m\u0131ndan Dogu\u000bAvrupa ve Akdeniz (C) genetik soyu i\u00e7erisinde degerlendirilmistir. Ayr\u0131ca COI-COII\u000bintergenik b\u00f6lgenin DNA dizi analizi sonucunda C1a, C1b, C1c, C1d, C1e, C1f, C1g ile C2a,\u000bC2e, C2f ve C2g olmak \u00fczere toplam 11 haplotip belirlenmistir. Bu haplotiplerden C1b, C1c,\u000bC1d, C1e, C1f, C1g ve C2f ile ifade edilen toplam 7 haplotip ilk defa bu arast\u0131rma sonucunda\u000btespit edilmistir.
\n<\/strong><\/p>\n

Anahtar Kelimeler:<\/strong> Apis mellifera L., mtDNA, PCR-RFLP, DNA dizi analizi, haplotip<\/p>\n","protected":false},"excerpt":{"rendered":"

Fulya \u00d6zdil Doktora Tezi, Ankara \u00dcniversitesi \/ Fen Bilimleri Enstit\u00fcs\u00fc \/ Zootekni Anabilim Dal\u0131, 2007, 141 s. TEZ NO: 213800<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[36],"tags":[154,145],"class_list":["post-17540","post","type-post","status-publish","format-standard","","category-doktora-tezleri","tag-fulya-ozdil","tag-turkiye-ari-irklari","yazarlar-fulya-ozdil","anahtarkelimeler-balarisi","anahtarkelimeler-balarisi-genetik-ozellikler","anahtarkelimeler-balarisi-ozellikleri-karsilastirma","anahtarkelimeler-hablotip"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17540","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17540"}],"version-history":[{"count":1,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17540\/revisions"}],"predecessor-version":[{"id":17541,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17540\/revisions\/17541"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17540"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17540"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17540"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}