{"id":17508,"date":"2014-09-10T23:25:41","date_gmt":"2014-09-10T20:25:41","guid":{"rendered":"http:\/\/balarisi.org\/?p=17508"},"modified":"2015-03-30T22:41:25","modified_gmt":"2015-03-30T19:41:25","slug":"otoimmun-tiroid-hastaliklarinda-ari-sutu-royal-jellynun-otoimmunite-uzerindeki-etkinliginin-arastirilmasi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17508","title":{"rendered":"Otoimm\u00fcn tiroid hastal\u0131klar\u0131nda ar\u0131 s\u00fct\u00fc (royal jelly)&#8217;n\u00fcn otoimm\u00fcnite \u00fczerindeki etkinli\u011finin ara\u015ft\u0131r\u0131lmas\u0131"},"content":{"rendered":"<p><strong>Cihangir Erem<\/strong><br \/>\nDoktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2005, 100 s.<br \/>\nTEZ NO: 165166<br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tarama.jsp\" target=\"_blank\">Sorgu Penceresine TEZ NO&#8217;yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z <\/a><\/span><\/p>\n<p><strong>\u00d6ZET<br \/>\n<\/strong>Otoimm\u00fcn tiroid hastal\u0131klar\u0131 i\u00e7inde hipertiroidinin en s\u0131k g\u00f6r\u00fclen nedeni Basedow- Graves hastal\u0131\u011f\u0131d\u0131r. Graves hastal\u0131\u011f\u0131 etyolojisi tam olarak bilinmeyen organ spesifik otoimm\u00fcn bir hastal\u0131kt\u0131r. Hastal\u0131\u011f\u0131n patogenezinde en \u00f6nemli rol\u00fc TSHR Ab oynar. Bu antikorun TSH resept\u00f6rlerini a\u015f\u0131r\u0131 \u015f\u00fcmule etmesiyle hipertiroidi ve guatr meydana gelir. Graves hastal\u0131\u011f\u0131n\u0131n patogenezinde sitokinlerin rol\u00fc son y\u0131llarda yo\u011fun olarak ara\u015ft\u0131r\u0131lm\u0131\u015ft\u0131r. Ar\u0131 s\u00fct\u00fc, gen\u00e7, i\u015f\u00e7i bal anlar\u0131n\u0131n (Apis mellifera) hipofarengeal ve mandib\u00fclar bezlerinde yap\u0131lan ve salg\u0131lanan, lapa-jel k\u0131vam\u0131nda homojen bir maddedir. Ar\u0131 s\u00fct\u00fcn\u00fcn antit\u00fcm\u00f6r, antibakteryel, antiallerjik, antiinflamatuar, imm\u00fcnomod\u00fclat\u00f6r, antihiperkolesterolemik, ins\u00fclin benzeri ve yorgunlu\u011fa kar\u015f\u0131 farmakolojik \u00f6zellikleri oldu\u011fu bildirilmi\u015ftir. Biz bu \u00e7al\u0131\u015fmam\u0131zda Graves hastal\u0131\u011f\u0131nda ar\u0131 s\u00fct\u00fcn\u00fcn otoimm\u00fcnite \u00fczerindeki etkisini periferik kan lenfosit h\u00fccre k\u00fclt\u00fcr ortam\u0131nda de\u011ferlendirmeyi ve tedavi dozunu belirlemeyi ama\u00e7lad\u0131k. \u00c7al\u0131\u015fman\u0131n ilk a\u015famas\u0131nda ar\u0131 s\u00fct\u00fcn\u00fcn imm\u00fcnite \u00fczerindeki etkisinin (stim\u00fclasyon, inhibisyon ya da imm\u00fcnmod\u00fclasyon) \u00f6l\u00e7\u00fclmesinde etkin konsantrasyonu bulmak i\u00e7in 4 normal sa\u011fl\u0131kl\u0131 bireyde periferik kandan lenfosit h\u00fccre izolasyonu yap\u0131ld\u0131. Ar\u0131 s\u00fct\u00fcn\u00fcn 0- 500 mg\/n\u0131l aral\u0131kta dil\u00fcsyonlar\u0131 haz\u0131rland\u0131. Her bir sa\u011fl\u0131kl\u0131 bireyin lenfositlerini i\u00e7eren izolasyon \u00f6rne\u011fine de\u011fi\u015fik konsantrasyonlardaki an s\u00fct\u00fc \u00f6rnekleri ilave edildi. 72 saatlik ink\u00fcbasyon sonras\u0131nda MTT testi yap\u0131ld\u0131. \u00c7al\u0131\u015fman\u0131n ikinci a\u015famas\u0131nda K.T.\u00dc. T\u0131p Fak\u00fcltesi \u0130\u00e7 Hastal\u0131klar\u0131 Anabilim Dali, Endokrinoloji ve Metabolizma Hastal\u0131klan Bilim Dal\u0131 Poliklini\u011fine ba\u015fvuran, klinik ve laboratuar bulgular\u0131yla yeni tan\u0131 konmu\u015f ve hi\u00e7\u000btedavi almam\u0131\u015f Graves&#8217;li 6 hasta \u00e7al\u0131\u015fmaya dahil edildi. Bu hastalarda periferik kan lenfosit izolasyonunu takiben O, 25 ve 400 mg\/ml&#8217;lik konsantrasyonlardaki an s\u00fct\u00fc \u00f6rnekleri hastalardan elde edilen lenfositlerle 72 saat s\u00fcreyle ink\u00fcbe edildi. Ink\u00fcbasyon sonras\u0131 lenfosit h\u00fccre k\u00fclt\u00fcr\u00fcnde MTT testi, h\u00fccre k\u00fclt\u00fcr supernatanlannda ise Thl sitokinlerinden IFN-y, TNF-ct ve \u0130L-12, Th2 sitokinlerinden IL-4 ve \u0130L-10 d\u00fczeyleri EASIA y\u00f6ntemiyle, TSHR Ab d\u00fczeyleri ise radyoresept\u00f6r y\u00f6ntemiyle tayin edildi. An s\u00fct\u00fcn\u00fcn Graves&#8217;li hastalar\u0131n periferik kan lenfosit h\u00fccre k\u00fclt\u00fcrlerinde 72 saatlik ink\u00fcbasyonu sonras\u0131nda h\u00fccresel toksisiteye neden olmayacak etkin konsantrasyonlar\u0131 MTT testi ile belirlendi ve lenfositleri en fazla etkileyen, proliferasyon yetene\u011fini en fazla art\u0131ran konsantrasyonun 400 mg\/ml oldu\u011fu bulundu. Ar\u0131 s\u00fct\u00fc konsantrasyonu artarken lenfositlerden \u00fcretilen ve salg\u0131lanan sitokinlerden IFN-y art\u0131\u015f g\u00f6sterirken; TNF-a, \u0130L-12, IL-4 ve \u0130L-10 azald\u0131. Sitokinlerden an s\u00fct\u00fc konsantrasyonuna g\u00f6re anlaml\u0131 farkl\u0131l\u0131klar IFN-y ve TNF-a&#8217;da bulundu. IL-4 konsantrasyonlan da anlaml\u0131l\u0131k s\u0131n\u0131r\u0131nda kald\u0131. Graves hastal\u0131\u011f\u0131n\u0131n patogenezinde, aktivasyon ve remisyon d\u00f6nemlerinde \u00f6nemli bir g\u00f6sterge olan Thl\/Th2 sitokin oranlar\u0131 i\u00e7erisinde IFN-y\/IL-4 ve IFN-y\/IL-10 oranlan da an s\u00fct\u00fc konsantrasyonlar\u0131na g\u00f6re anlaml\u0131 farkl\u0131l\u0131klar g\u00f6sterdi. Ar\u0131 s\u00fct\u00fc Graves&#8217;li hastalarda Thl\/Th2 sitokin oran\u0131n\u0131 Thl sitokin y\u00f6n\u00fcne kayd\u0131rd\u0131. Dolay\u0131s\u0131yla an s\u00fct\u00fc kullan\u0131m\u0131 Graves tedavisinde ve remisyon sa\u011flanmas\u0131nda AT\u0130&#8217;lara benzer \u015fekilde etkili olabilir. Ar\u0131 s\u00fct\u00fc ile muamele edilen lenfositlerden TSHR Ab d\u00fczeyleri ar\u0131 s\u00fct\u00fc konsantrasyonu artt\u0131k\u00e7a belirgin anlaml\u0131 azalmalar g\u00f6sterdi. Bu sonu\u00e7 ar\u0131 s\u00fct\u00fcn\u00fcn TSHR Ab d\u00fczeylerini d\u00fc\u015f\u00fcrmede bir AT\u0130 gibi etki edebilece\u011fini g\u00f6stermektedir. Gerek sitokin ve gerekse TSHR Ab d\u00fczeylerini etkileyen ve farkl\u0131l\u0131k meydana getiren ar\u0131 s\u00fct\u00fc konsantrasyonu 400 mg\/ml olarak bulundu. Sonu\u00e7 olarak, ar\u0131 s\u00fct\u00fcn\u00fcn Graves hastal\u0131\u011f\u0131nda imm\u00fcnmod\u00fclat\u00f6r etkili olabilece\u011fi sonucuna var\u0131ld\u0131.\u000b<strong><br \/>\n<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cihangir Erem Doktora Tezi, Karadeniz Teknik \u00dcniversitesi \/ Sa\u011fl\u0131k Bilimleri Enstit\u00fcs\u00fc \/ Biyokimya Anabilim Dal\u0131, 2005, 100 s. TEZ NO: 165166<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[36],"tags":[163,307,306],"class_list":["post-17508","post","type-post","status-publish","format-standard","","category-doktora-tezleri","tag-apiterapi-2","tag-ari-sutu","tag-cihangir-erem","yazarlar-cihangir-erem","anahtarkelimeler-apiterapi","anahtarkelimeler-ari-sutu","anahtarkelimeler-ari-sutunun-faydalari","anahtarkelimeler-tiroid-hastaligi"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17508","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17508"}],"version-history":[{"count":3,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17508\/revisions"}],"predecessor-version":[{"id":17511,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17508\/revisions\/17511"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17508"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17508"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}