{"id":17269,"date":"2014-07-06T22:49:05","date_gmt":"2014-07-06T19:49:05","guid":{"rendered":"http:\/\/balarisi.org\/?p=17269"},"modified":"2015-03-31T16:03:18","modified_gmt":"2015-03-31T13:03:18","slug":"cesitli-bal-arisi-apis-mellifera-l-populasyonlarinda-rapd-rasgele-cogaltilmis-polimorfik-dna-polimorfizmi","status":"publish","type":"post","link":"https:\/\/www.beyazkovan.com\/?p=17269","title":{"rendered":"\u00c7e\u015fitli bal ar\u0131s\u0131 (Apis mellifera L.) populasyonlar\u0131nda RAPD (Rasgele \u00e7o\u011falt\u0131lm\u0131\u015f polimorfik DNA) Polimorfizmi"},"content":{"rendered":"<p><strong> <a href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ&amp;keyword=YILDIZ%2C%20Mehmet%20Ali&amp;s_f=3\">YILDIZ, Mehmet Ali<\/a>; <a href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ&amp;keyword=FIRATLI%2C%20A.%20%C7etin&amp;s_f=3\">FIRATLI, A. \u00c7etin<\/a>; <a href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ&amp;keyword=BA%DEPINAR%2C%20Ensar&amp;s_f=3\">BA\u015ePINAR, Ensar<\/a>; <a href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ&amp;keyword=GEN%C7ER%2C%20H.%20Vasfi&amp;s_f=3\">GEN\u00c7ER, H. Vasfi<\/a>; <a href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ&amp;keyword=%D6ZD%DDL%2C%20Fulya&amp;s_f=3\">\u00d6ZD\u0130L, Fulya<\/a><\/strong><br \/>\nT\u00dcB\u0130TAK VHAG Proje 2046 103V070, 2006: 1-33<br \/>\nMakale No:\u00a0<strong>72118<\/strong><br \/>\n<!--more--><span style=\"color: #ff6600;\"><a style=\"color: #ff6600;\" href=\"http:\/\/uvt.ulakbim.gov.tr\/uvt\/index.php?cwid=3&amp;vtadi=TPRJ\" target=\"_blank\">Sorgu Penceresine MAKALE NO\u2019yu yazarak Ayr\u0131nt\u0131l\u0131 Bilgi ve PDF d\u00f6k\u00fcmana ula\u015fmak i\u00e7in t\u0131klay\u0131n\u0131z<\/a><\/span><\/p>\n<p style=\"-qt-block-indent: 0; text-indent: 0px; margin: 0px;\"><strong>\u00d6ZET<\/strong><br \/>\nBu ara\u015ft\u0131rmada, T\u00fcrkiye&#8217;nin farkl\u0131 b\u00f6lgelerinden \u00f6rneklenen bal ar\u0131s\u0131 populasyonlar\u0131n\u0131n genetik yap\u0131lar\u0131, RAPD (rasgele \u00e7o\u011falt\u0131lm\u0131\u015f polimorfik DNA} markerleri kullan\u0131larak populasyonlar i\u00e7i ve popuiasyonlar aras\u0131 genetik benzerlikler (ya da farkl\u0131l\u0131klar) tespit edilmi\u015ftir. K\u00fcmeleme analizinden yararlan\u0131larak bal ans\u0131 populasyonlan aras\u0131ndaki filogenetik ili\u015fkiler belirlenmi\u015ftir. Elde edilen bulgular \u0131\u015f\u0131\u011f\u0131nda, gezginci anal\u0131\u011f\u0131n T\u00fcrkiye bal ar\u0131s\u0131 populasyonlar\u0131ndaki mevcut genetik \u00e7e\u015fitlilik \u00fczerine olas\u0131 etkileri tart\u0131\u015f\u0131lm\u0131\u015ft\u0131r. Ara\u015ft\u0131rmada T\u00fcrkiye&#8217;nin 10 farkl\u0131 b\u00f6lgesinden \u00f6rneklenen toplam 100 kolonide 20 RAPD primeri bak\u0131m\u0131ndan toplam 149 lokus (1381 polimorfik) tespit edilerek primer ba\u015f\u0131na ortalama polimorfik lokus say\u0131s\u0131 6,9 (138\/20) olarak hesaplanm\u0131\u015ft\u0131r. Bal ar\u0131s\u0131 populasyoniar\u0131ndaki genetik varyasyonun ortaya konulmas\u0131 i\u00e7in yararlan\u0131lan ve t\u00fcm allel frekanslar\u0131 \u00fczerinden hesaplanan ortalama allel say\u0131s\u0131 (% =1.926), ortalama etkili allel say\u0131s\u0131 (na= 1.568), beklenen ortalama neterozigotiuk (hj =0.235), ortalama heterozigotluk (tf =0.331), Shannon sabiti (Ho =0,493), polimorfik lokus say\u0131s\u0131 (np =138) ve polimorfik lokus oran\u0131 (Ppoly =%92.62) olduk\u00e7a y\u00fcksek bulunmu\u015ftur. Bal ar\u0131s\u0131 populasyonlar\u0131 aras\u0131ndaki genetik farkl\u0131l\u0131klar\u0131n ortaya konulmas\u0131nda kullan\u0131lan genetik farkl\u0131la\u015fma katsay\u0131s\u0131 (%r), 0.2889 olarak hesaplanm\u0131\u015ft\u0131r. Populasyonlar i\u00e7i ortalama heterozigotluk (%) 0.2346, toplam heterozigottuk {Kr) ise 0.3299 olarak tahmin edilmi\u015ftir. Populasyonlar\u0131 birbirlerinden olan farkl\u0131la\u015fmalar\u0131n\u0131 g\u00f6steren genetik farkl\u0131la\u015fma katsay\u0131s\u0131ndan yararlan\u0131larak tespit edilen toplam genetik varyasyonun % 71.111nin populasyonlar i\u00e7erisinde, geri kalan k\u0131sm\u0131n (% 28.89) ise populasyonlar aras\u0131nda oldu\u011fu ortaya konulmu\u015ftur. Bal ar\u0131s\u0131 populasyonlar\u0131 aras\u0131nda her generasyon g\u00f6\u00e7 eden birey say\u0131s\u0131 (gen ak\u0131\u015f\u0131) Nm ortalama 1.2301 olarak hesaplanm\u0131\u015ft\u0131r. Populasyonlar aras\u0131ndaki genetik mesafe (D) de\u011ferleri 0,0716 ile 0.2283 aras\u0131nda hesaplanm\u0131\u015ft\u0131r. Sonu\u00e7 olarak, T\u00fcrkiye bal ans\u0131 populasyonlar\u0131n\u0131n kendi i\u00e7lerinde ayn\u0131 d\u00fczeyde y\u00fcksek genetik varyasyon g\u00f6sterdikleri, \u00e7al\u0131\u015f\u0131lan pulasyonlar\u0131 birbirlerinden \u00f6nemli \u00f6l\u00e7\u00fcde farkl\u0131la\u015ft\u0131klar\u0131, populasyonlar aras\u0131nda az say\u0131da g\u00f6\u00e7\u00fcn meydana geldi\u011fi, populasyonlann orijin ald\u0131klar\u0131 farkl\u0131 genotip gruplar\u0131na g\u00f6re s\u0131n\u0131fland\u0131r\u0131lmas\u0131n\u0131n olduk\u00e7a zor oldu\u011fu ve gezginci anal\u0131\u011f\u0131n genetik varyasyonu art\u0131r\u0131c\u0131 y\u00f6nde bir etkiye sahip oldu\u011fu tespit edilmi\u015ftir.<\/p>\n<p style=\"-qt-block-indent: 0; text-indent: 0px; margin: 0px;\"><strong>Anahtar Kelimeler:<\/strong> Apis mellifera; Bal ar\u0131s\u0131; Ekotip; Genetik varyasyon; RAPD<\/p>\n","protected":false},"excerpt":{"rendered":"<p>YILDIZ, Mehmet Ali; FIRATLI, A. \u00c7etin; BA\u015ePINAR, Ensar; GEN\u00c7ER, H. Vasfi; \u00d6ZD\u0130L, Fulya T\u00dcB\u0130TAK VHAG Proje 2046 103V070, 2006: 1-33 Makale No:\u00a072118<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"footnotes":""},"categories":[33],"tags":[40,104,50,153,154,78,72,109],"class_list":["post-17269","post","type-post","status-publish","format-standard","","category-tubitak-destekli-projeler","tag-cetin-firatli","tag-dna","tag-ekotip","tag-ensar-baspinar","tag-fulya-ozdil","tag-genetik-cesitlilik","tag-h-vasfi-gencer","tag-mehmet-ali-yildiz","yazarlar-cetin-firatli","yazarlar-ensar-baspinar","yazarlar-fulya-ozdil","yazarlar-h-vasfi-gencer","yazarlar-mehmet-ali-yildiz","anahtarkelimeler-balarisi","anahtarkelimeler-balarisi-genetik-cesitlilik","anahtarkelimeler-balarisi-genetik-ozellikler","anahtarkelimeler-ekotip"],"_links":{"self":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17269","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=17269"}],"version-history":[{"count":3,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17269\/revisions"}],"predecessor-version":[{"id":17271,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=\/wp\/v2\/posts\/17269\/revisions\/17271"}],"wp:attachment":[{"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=17269"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=17269"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.beyazkovan.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=17269"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}